Variation of the phenolic composition and a-glucosidase inhibition potential of seeds, soaked seeds, and sprouts of four wild forms and four varieties of common bean (Phaseolus vulgaris)

The determination of the changes in the composition of bioactive phenolic compounds of germinating seeds which accumulate high levels of these compounds could contribute to the understanding of the germination mechanism and the development of markers for the selection of plant genotypes. In the current study, the changes in the phenolic composition and a-glucosidase inhibition activity, taking place during the germination of four wild forms and four varieties of common bean (Phaseolus vulgaris L.) from Durango Mexico, were determined. A total of 66 phenolic compounds (19 phenolic acids, 18 isoflavones, 18 flavonol glycosides, 3 flavonol aglycones, 3 flavones, 2 dihydroflavonoids, 2 chalcones and one non-identified type) were found by HPLC-DAD, which were differentially accumulated by the seeds, 24 h-soaked seeds, and 4 day-sprouts of each genotype. The accumulation of the flavonol aglycones, myricetin, quercetin and kaempferol was distinctive of the wild seeds. Soaking not only caused leaching and degradation but also triggered the synthesis of new phenolic compounds whereas germination diversified the composition of isoflavones and flavonol glycosides. The seeds of all genotypes analyzed were important inhibitors of a-glucosidase, improving their potential after soaking and germination. The results suggested that the structure rather than the concentration of the flavonoids and phenolic acids determined the inhibitory potential of a-glucosidase of samples. The principal component analysis and cluster analysis revealed HPLC-DAD phenolic profiles as genotype-specific chemomarkers at any of the states (seeds, soaked seeds, and sprouts). The results have wide implications on agronomy and food quality

Epidermolysis bullosa simplex with mottled pigmentation: a family report and review

Epidermolysis bullosa simplex with mottled hyperpigmentation (EBS-MP) is an uncommon subtype of EBS. Its clinical features depend on the age of diagnosis, and clinical variations have been described even within family members. We present six cases from two unrelated Spanish families each with several affected members with EBS-MP and review the clinical and genetic findings in all reported patients. We highlight the changing clinical features of the disease throughout life

Evaluation of Burnout Syndrome and Resilience in Jaén Physical Therapists during the COVID-19 Pandemic. Pilot Study

Artículos originales[ES] Introducción: Aunque el síndrome del quemado (burnout) se ha estudiado profusamente en los trabajadores sanitarios,existen pocos estudios que lo aborden específicamente en los fisioterapeutas y cuando esto se ha hecho se ha encontrado que los índices varían desde un nivel bajo hasta un nivel medio-alto. La reciente pandemia COVID-19 ha supuesto y supone un reto notable para el sistema sanitario. En este escenario, puede ser de interés evaluar el índice de burnout en el colectivo de fisioterapeutas.Objetivo: El objetivo de este estudio es medir la prevalencia de burnout en el colectivo de fisioterapeutas de Jaén y provincia, así como estudiar posibles fenómenos asociados a la misma. Material y Métodos: Se remitió una encuesta con datos sociodemográficos, una escala de medición de la resiliencia y una escala de medición del burnout (incluyendo sus tres aspectos: cansancio emocional, despersonalización y realización personal) por medio grupos de WhatsApp de ámbito profesional a los fisioterapeutas de la provincia de Jaén. La encuesta fue anónima, voluntaria y autocumplimentable. Resultados: La prevalencia de burnout entre los fisioterapeutas de la provincia de Jaén es superponible a la publicada en otros estudios. Los fisioterapeutas que no trabajan en la capital sufren más de cansancio emocional (p=0,038) y existe una tendencia a que los hombres sufran más despersonalización. Entre las subescalas de resiliencia que más se asocian al burnout está la de “adaptabilidad y redes de apoyo”.Conclusiones: La frecuencia de burnout en los fisioterapeutas de Jaén es superponible a la publicada por otros autores, no siendo posible establecer si el contexto de pandemia COVID juega algún papel. No trabajar en la capital de la provincia parece favorecer el burnout. El perfil de burnout se concreta sobre todo en los aspectos “cansancio emocional” y “despersonalización”, siendo esto especialmente frecuente en los que presentan un bajo nivel de resiliencia en la subescala “adaptabilidad y redes de apoyo” [EN] Introduction: Although burnout syndrome has been extensively studied in health care workers, there are few studies that specifically address it in physical therapists, and when this has been done, the rates have been found to range from low to medium-high. The recent COVID-19 pandemic has been and continues to be a significant challenge for the healthcare system. In this scenario, it may be of interest to evaluate the burnout index in the group of physical therapists. Objective: The objective of this study is to measure the prevalence of burnout in the group of physical therapists in Jaén and province, as well as to study possible phenomena associated with it. Material and Methods: A survey with sociodemographic data, a resilience measurement scale and a burnout measurement scale (including its three aspects: emotional exhaustion, depersonalization and personal fulfillment) was sent by means of WhatsApp groups of professional scope to the physical therapists of the province from Jaén. The survey was anonymous, voluntary, and self-filling.Results: The prevalence of burnout among physiotherapists in the province of Jaén is superimposable to that published in other studies. Physiotherapists who do not work in the capital suffer more from emotional fatigue (p = 0.038) and there is a tendency for men to suffer more depersonalization. Among the resilience subscales that are most asso ciated with burnout is “adaptability and support networks”. Conclusions: The frequency of burnout in Jaén physiotherapists is superimposable to that published by other authors, and it is not possible to establish whether the context of the COVID pandemic plays any role. Not working in the provincial capital seems to favor burnout. The burnout profile is specified above all in the “emotional fatigue” and “depersonalization” aspects, this being especially frequent in those with a low level of resilience in the “adaptability and support networks” subscale.N

Effect of ABCB1 and ABCC3 Polymorphisms on Osteosarcoma Survival after Chemotherapy: A Pharmacogenetic Study

Standard treatment for osteosarcoma patients consists of a combination of cisplatin, adriamycin, and methotrexate before surgical resection of the primary tumour, followed by postoperative chemotherapy including vincristine and cyclophosphamide. Unfortunately, many patients still relapse or suffer adverse events. We examined whether common germline polymorphisms in chemotherapeutic transporter and metabolic pathway genes of the drugs used in standard osteosarcoma treatment may predict treatment response. METHODOLOGY/PRINCIPAL FINDINGS: In this study we screened 102 osteosarcoma patients for 346 Single Nucleotide Polymorphisms (SNPs) and 2 Copy Number Variants (CNVs) in 24 genes involved in the metabolism or transport of cisplatin, adriamycin, methotrexate, vincristine, and cyclophosphamide. We studied the association of the genotypes with tumour response and overall survival. We found that four SNPs in two ATP-binding cassette genes were significantly associated with overall survival: rs4148416 in ABCC3 (per-allele HR = 8.14, 95%CI = 2.73-20.2, p-value = 5.1x10(-)(5)), and three SNPs in ABCB1, rs4148737 (per-allele HR = 3.66, 95%CI = 1.85-6.11, p-value = 6.9x10(-)(5)), rs1128503 and rs10276036 (r(2) = 1, per-allele HR = 0.24, 95%CI = 0.11-0.47 p-value = 7.9x10(-)(5)). Associations with these SNPs remained statistically significant after correction for multiple testing (all corrected p-values [permutation test] </= 0.03). CONCLUSIONS: Our findings suggest that these polymorphisms may affect osteosarcoma treatment efficacy. If these associations are independently validated, these variants could be used as genetic predictors of clinical outcome in the treatment of osteosarcoma, helping in the design of individualized therapy

Changes in Life History Traits of Small Pelagic Fish in the Western Mediterranean Sea

21 pages, 8 figures, 3 tables, supplementary material https://www.frontiersin.org/articles/10.3389/fmars.2021.570354/full#supplementary-material.-- Data Availability Statement: The datasets presented in this article are not readily available because the dataset is available by request to the Spanish Institute of Oceanography. Requests to access the datasets should be directed to [email protected] pelagic fish (SPF) in the western Mediterranean Sea are key elements of the marine food web and are important in terms of biomass and fisheries catches. Significant declines in biomass, landings, and changes in the age/size structure of sardine Sardina pilchardus and anchovy Engraulis encrasicolus have been observed in recent decades, particularly in the northern area of the western Mediterranean Sea. To understand the different patterns observed in SPF populations, we analyzed key life history traits [total length at age, length at maturity, gonadosomatic index (GSI), and body condition (Kn)] of sardine and anchovy collected between 2003 and 2017, from different fishing harbors distributed along a latitudinal gradient from northern to southern Spain. We used Generalized Linear Models (GLM) to estimate the length at maturity and Generalized Additive Models (GAMs) to test the relationship with environmental variables (seawater temperature, water currents, and net primary productivity). The life history traits of both species presented seasonal, interannual and latitudinal differences with a clear decline in length at age, length at first maturity, and body condition, for both species in the northern part of the study area. In the southern part, on the contrary, life history traits did not present a clear temporal trend. The environmental conditions partially explained the long-term changes in life history traits, but the selected variables differed between areas, highlighting the importance of regional oceanographic conditions to understand the dynamics of small pelagic fish. The truncated length-at-age pattern for both species with the disappearance of the larger individuals of the population could have contributed to the poor condition of small pelagic fish populations in the northern part of the western Mediterranean Sea in recent years. In the south area, recent declines in body condition for sardine and anchovy were observed and could be a possible first sign for future population declines. This study highlights the importance of understanding the trade-off between the energy invested in reproduction, maintenance and growth at seasonal and interannual level to advance our knowledge on how environmental and human pressures influence population dynamics of small pelagic fish at local and regional scalesThis study was carried out within the Spanish Research project PELWEB (CTM2017-88939-R) funded by Spanish Ministry of Science, Innovation and Universities and the European Research Contract SPELMED (SC NR 02-TENDER EASME/EMFF/2016/032XXX) funded by EC EASME. Fisheries data collection has been co-funded by the EU through the European Maritime and Fisheries Fund (EMFF) within the National Program of collection, management and use of data in the fisheries sector and support for scientific advice regarding the Common Fisheries Policy (Regulation, EU 2017/1004)With funding from the Spanish government through the ‘Severo Ochoa Centre of Excellence’ accreditation (CEX2019-000928-S)Peer reviewe

Effect of ABCB1 and ABCC3 Polymorphisms on Osteosarcoma Survival after Chemotherapy: A Pharmacogenetic Study

Background: Standard treatment for osteosarcoma patients consists of a combination of cisplatin, adriamycin, and methotrexate before surgical resection of the primary tumour, followed by postoperative chemotherapy including vincristine and cyclophosphamide. Unfortunately, many patients still relapse or suffer adverse events. We examined whether common germline polymorphisms in chemotherapeutic transporter and metabolic pathway genes of the drugs used in standard osteosarcoma treatment may predict treatment response. Methodology/Principal Findings: In this study we screened 102 osteosarcoma patients for 346 Single Nucleotide Polymorphisms (SNPs) and 2 Copy Number Variants (CNVs) in 24 genes involved in the metabolism or transport of cisplatin, adriamycin, methotrexate, vincristine, and cyclophosphamide. We studied the association of the genotypes with tumour response and overall survival. We found that four SNPs in two ATP-binding cassette genes were significantly associated with overall survival: rs4148416 in ABCC3 (per-allele HR = 8.14, 95%CI = 2.73-20.2, p-value = 5.1×10 -5), and three SNPs in ABCB1, rs4148737 (per-allele HR = 3.66, 95%CI = 1.85-6.11, p-value = 6.9×10 -5), rs1128503 and rs10276036 (r 2 = 1, per-allele HR = 0.24, 95%CI = 0.11-0.47 p-value = 7.9×10 -5). Associations with these SNPs remained statistically significant after correction for multiple testing (all corrected p-values [permutation test] ≤0.03). Conclusions: Our findings suggest that these polymorphisms may affect osteosarcoma treatment efficacy. If these associations are independently validated, these variants could be used as genetic predictors of clinical outcome in the treatment of osteosarcoma, helping in the design of individualized therapyThis work was supported by the AECC (Asociación Española contra el Cáncer), FIS (Fondo de Investigación Sanitaria-Instituto de Salud Carlos III) and the ‘‘Inocente Inocente’’ Foundatio

Optimizando el abordaje del asma bronquial

196 páginas.El asma bronquial es una enfermedad crónica que afecta a millones de personas en todo el mundo, siendo la primera enfermedad crónica en la edad pediátrica. Se caracteriza por una inflamación de las vías respiratorias que conduce a la obstrucción del flujo de aire y a la dificultad para respirar. El asma es una enfermedad que afecta a la calidad de vida de las personas que la padece y puede ser una condición potencialmente mortal si no se trata adecuadamente. A pesar de los avances significativos en el tratamiento del asma en las últimas décadas, todavía hay muchas personas que no logran controlar adecuadamente su enfermedad. Muchos pacientes continúan experimentando síntomas a pesar de tomar medicamentos y seguir las recomendaciones médicas. Esto puede deberse a multitud de factores, como una mala adherencia al tratamiento, una falta de comprensión de la enfermedad o una falta de seguimiento por parte de los profesionales sanitarios. Una formación adecuada de los profesionales sanitarios implicados en la atención al paciente asmático (Médicos de Familia, Médicos de urgencias, Pediatras, Neumólogos, Alergólogos, etc) ayudará a establecer una estrategia de tratamiento eficaz y eficiente que pueda traducirse en una mejora del control y por tanto de la calidad de vida de nuestros pacientes. En este libro, se abordan las últimas recomendaciones basadas en la evidencia para el diagnóstico, la evaluación y el tratamiento del asma, con un enfoque multidisciplinar. A lo largo de sus diez capítulos, se describe como llegar a establecer un diagnostico correcto del asma (interpretación de pruebas alérgicas, espirometria y técnicas de imagen), uso adecuado de los distintos dispositivos de inhalación, las peculiaridades del tratamiento en el embarazo y en la edad pediátrica, el importante papel de la inmunoterapia en el tratamiento del asma alérgica, así de como debemos actuar ante una crisis de asma o cuales son los avances actuales y futuros en el asma grave o de difícil control

La influencia de la tecnología en los procesos de producción musical: análisis de la grabación sonora y sus aplicaciones en el ámbito docente

Memoria de las actividades del proyecto de Innova-Docencia nº256, coordinado por el Dr. Marco Antonio Juan de Dios Cuartas, profesor del Departamento de Musicología de la Universidad Complutense de Madrid

El laboratorio sonoro como herramienta docente para el análisis de la creación e interpretación musical a través de las grabaciones

Depto. de MusicologíaFac. de Geografía e HistoriaFALSEsubmitte

Towards biological and ecological knowledge of Lophius spp. in the NW Mediterranean Sea for a sustainable fishery

[eng] In the northwestern Mediterranean Sea, anglerfishes Lophius budegassa Spinola, 1807 and Lophius piscatorius Linnaeus, 1758 are among the most valuable demersal species and support an important fishery. Both anglerfishes are mainly caught in mixed bottom trawl fisheries operating in the continental shelf and upper slope. Their landings, which are primarily composed of L. budegassa (86%) and a small percentage of L. piscatorius (14%), have increased lately with the development of directed fisheries reaching 9,486 t with a value of 65 million of Euros over the period 2000-2016. Up to date, European Commission has been conducted stock assessment for L. budegassa inhabiting Mediterranean waters but no effort has been made to assess L. piscatorius. Due to the commercial importance of both Lophius species, the aim of this Thesis is to improve the knowledge of the population parameters of L. budegassa and L. piscatorius of the northwestern Mediterranean Sea in order to determine the state of exploitation of both species and establish an effectively management. To achieve this aim, a set of specific objectives are addressed: to determine the behavioural activity and sensory mechanisms for reducing the levels of their ecological competition; to describe the reproductive parameters for a better understanding of the life cycle of both Lophius spp.; to examine morphological characteristics of ova in the two species of Lophius for aid in identification of eggs from ichthyoplankton samples; to provide new data on population dynamics as well as on population structure and biometric relationships for modelling the stock of both Lophius spp.; to explore the sustainability indicators for monitoring the state of Lophius population relative to exploitation; and to analyse the prevalence of the microsporidian parasite Spraguea lophii for hypothesize about the health status of the population of Lophius spp. and the possible side effects of severe infections on their morphology. The results demonstrate that although L. budegassa and L. piscatorius occupy the same habitat in the northwestern Mediterranean Sea, differences noted in their life history traits support the hypothesis that both species of Lophius have different ecological strategies in order to lessen competition between them. According to the differences found in the life history traits for both species, it is highly recommended to carry out a differentiated management for each Lophius species in the northwestern Mediterranean Sea. In conclusion, this Thesis improves the current understanding of the biology and ecology of L. budegassa and L. piscatorius; and it is the first study to provide information on the reproductive strategy of L. piscatorius in the northwestern Mediterranean Sea; contributing to increase the scientific knowledge of both Lophius spp. which will be useful to improve future stock assessments and ensure proper management actions[spa] En el noroeste del mar Mediterráneo, las especies de rape Lophius budegassa Spinola, 1807 y Lophius piscatorius Linnaeus, 1758 se encuentran entre las especies demersales más valiosas sobre las cuales se efectúa una actividad pesquera importante. Ambas especies son capturadas generalmente en pesquerías mixtas de arrastre de fondo que operan en la plataforma continental y en el talud superior. Las descargas se componen principalmente de L. budegassa (86%) y un pequeño porcentaje de L. piscatorius (14%), y han aumentado en los últimos años debido al desarrollo de una pesca más dirigida, alcanzando 9,486 t y un valor de 65 millones de euros durante el período 2000-2016. Hasta la fecha, la Comisión Europea ha realizado evaluaciones de las poblaciones de L. budegassa que habitan las aguas del Mediterráneo, pero no se ha hecho ningún esfuerzo respecto a L. piscatorius. Atendiendo a la importancia comercial de estas especies, el objetivo de esta Tesis ha sido mejorar el conocimiento de los parámetros de la población de L. budegassa y L. piscatorius del noroeste del Mar Mediterráneo para determinar su estado de explotación y establecer una gestión efectiva. Para alcanzar dicho objetivo, se plantearon un conjunto de objetivos específicos: determinar su comportamiento y los mecanismos sensoriales para reducir los niveles de competencia ecológica; describir los parámetros reproductores para mejorar la comprensión del ciclo de vida; examinar las características morfológicas de los óvulos para identificar los huevos a partir de muestras de ictioplancton; proporcionar nuevos datos sobre la dinámica de la población, así como sobre la su estructura y las relaciones biométricas para modelar los stocks; explorar los indicadores de sostenibilidad para monitorear el estado de la población en relación con la explotación; y analizar la prevalencia del parásito microspordio Spraguea lophii para formular hipótesis sobre el estado de salud de la población de Lophius y los posibles efectos secundarios de infecciones graves en su morfología. Los resultados han demostrado que L. budegassa y L. piscatorius ocupan el mismo hábitat en el noroeste del Mediterráneo, si bien las diferencias observadas en sus parámetros del ciclo de vida apoyan la hipótesis de que ambas especies tienen diferentes estrategias ecológicas para disminuir la competencia entre ellas. De acuerdo con las diferencias encontradas, se recomienda realizar una gestión diferenciada para cada especie de Lophius en el noroeste del Mar Mediterráneo